Protocols

FACS Staining Related Protocols

• SURFACE STAIN Basic staining protocol for flow cytometry - direct and indirect labeling of surface markers: https://stanfordmedicine.box.com/v/FACS-Surface-Stain

• ANTIBODY TITRATION Overview and example of antibody titration to determine optimal antibody concentations to use:  https://stanfordmedicine.box.com/v/Antibody-Titration

• VIABILITY STAINS Overview of Cell Viability stains and example protocols:  https://stanfordmedicine.box.com/v/Cell-Viability-Staining

• FMOs Gating controls - what is an FMO (Fluorescence Minus One) controls and how to use it:  https://stanfordmedicine.box.com/v/Using-FMO-controls

• FIXATION PROTOCOL Basic Fixation protocol for Stained samples, steps for formaldehyde fixation - https://stanfordmedicine.box.com/v/Basic-Fixation

Instrument Related Protocols

• coming soon