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Available Instruments

Consultation- Main Facility

Analyzers

Abbott

PDF icon Abbott Configuration

Abbott is a 3-laser, 17-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
1651 Page Mill
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
405nm, 488nm, 633nm

Abbott Request Only

PDF icon abbottsept18-compatibilitymode.pdf

Abbott is a 3-laser, 17-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD LSR ll
Room: 
1651 Page Mill
Maximum cells/second: 
10000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
405nm, 488nm, 633nm

Aurora

3-laser (Blue-Violet-Red) spectral cytometer capable of  analyzing >20-color panels

Model: 
Aurora
Room: 
B016
Sort: 
No
Maximum cells/second: 
35000
BSL-2: 
Yes
Colors: 
>20
Laser Excitations: 
488, 405, 640

Aurora

5-laser (UltraViolet-Blue-Violet-Yello-Red) spectral cytometer capable of  analyzing >30-color panels

Model: 
Aurora
Room: 
B016
Sort: 
No
Maximum cells/second: 
3500
BSL-2: 
Yes
Colors: 
>35
Laser Excitations: 
355 405 488 561 640

LSR II

PDF icon LSR II Configuration

LSRII is a 4-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm

LSR II.2

PDF icon LSRII.2 configuration

LSRII.2 is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 6-Violet, 2-Blue, 4-Green, 3-UV, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

LSR II.UV

PDF icon LSRII-UV Configuration

LSRII.UV is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  It has recently been upgraded to support the latest violet and UV excited reagents.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 5-Yellow, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016D
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm

Quanteon

PDF icon Quanteon Configuration

4-laser, 25-fluorescent channels plus 2 scatter channels; excellent plate loader for both 96- and 384-well plates or up to 40 sample tubes

Model: 
Acea Novocyte Quanteon
Room: 
B016C
Sort: 
No
Maximum cells/second: 
20000
BSL-2: 
Yes
Colors: 
25
Laser Excitations: 
Blue, Violet, Yellow, Red

Quanteon Sample Drop-off

PDF icon Quanteon Configuration

4-laser, 25-fluorescent channels plus 2 scatter channels; excellent plate loader for both 96- and 384-well plates or up to 40 sample tubes

Model: 
Acea Novocyte Quanteon
Room: 
B003
Sort: 
No
Maximum cells/second: 
20000
BSL-2: 
Yes
Colors: 
25
Laser Excitations: 
Blue, Violet, Yellow, Red

Scanford

PDF icon Scanford Configuration

The FACScan Analyzer was one of the first successful bench top flow cytometers. They just keep going and going. This one has been upgraded to 4-laser, 12-color configuration.  Its current laser/detector configuration provides 2-Violet(co-linear with Red, 3-Red (co-linear with Violet), 3-Blue, and 4-Yellow laser excited detection channels.

Model: 
Custom FACScan
Room: 
B016D
Sort: 
No
Maximum cells/second: 
3,000
BSL-2: 
Yes
Colors: 
12
Laser Excitations: 
488nm, 405 nm, 561nm, 640nm

Symphony

BD FACS Symphony A5 is a  5-laser, 29-color instrument compatible with the 96-well plate sampler.  

Model: 
BD FACS Symphony A5
Room: 
B003
Sort: 
No
Maximum cells/second: 
10000
Colors: 
29
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

Mass Cytometer

Cyrano

The CyTOF2 Mass Cytometer uses metal-ion conjugates to label cells.  Labeled cells are then ionized and mass measurements are used to differentiate functional and phenotypic markers.  Sample preparation and processing is quite similar to that used in fluorescence-based cytometry with some quality control considerations unique to atomic mass spectroscopy. Please contact staff for further details.

Model: 
DVS CyTOF II
Room: 
B015A
Sort: 
No
Maximum cells/second: 
500
BSL-2: 
Yes
Colors: 
~100
Laser Excitations: 
N/A

Cyrano Request Only

The CyTOF2 Mass Cytometer uses metal-ion conjugates to label cells.  Labeled cells are then ionized and mass measurements are used to differentiate functional and phenotypic markers.  Sample preparation and processing is quite similar to that used in fluorescence-based cytometry with some quality control considerations unique to atomic mass spectroscopy. Please contact staff for further details.

Model: 
DVS CyTOF ll
Room: 
B015A
Sort: 
No
Maximum cells/second: 
500
BSL-2: 
Yes
Colors: 
~100
Laser Excitations: 
N/A

Sorters

Aida

Aida

PDF icon Aida Configuration

Aida is a 4-laser, 14-color sorter.  Its current laser/detector configuration provides 5-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
13
Laser Excitations: 
488nm, 561nm, 635nm, 405nm
Aria

Aria

PDF icon Aria Configuration

Aria is a 3-laser, 11-color sorter.  Its current laser/detector configuration provides 3-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
11
Laser Excitations: 
488nm, 635nm, 405nm
Aria

Aria Staff Assisted

PDF icon Aria Configuration

Aria is a 3-laser, 11-color sorter.  Its current laser/detector configuration provides 3-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD Aria ll
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
11
Laser Excitations: 
488nm, 635nm, 405nm
Carmen

Carmen

PDF icon Carmen Configuration

Carmen is a 6 laser sorter capable of up to 6 way sorting.  It is our fastest plate sorter; both 384- and 96- well plate sorts require only 1/3 the time needed on an Aria type sorter.  It is the only instrument with an 457nm excitation laser, making it ideal for fluorochromes such as CFP or mTurquoise.  It is available only with operator support.

Model: 
BD Influx Special Order
Room: 
B015A
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
355nm, 405nm, 457nm, 488nm, 561nm, 640nm

Costello

PDF icon Costello Configuration

Costello is a 5 laser, 18-color Aria Fusion sorter.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
1651 Page Mill
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm

Costello Request Only

PDF icon costellosept18-compatibilitymode.pdf

Costello is a 5 laser, 18-color Aria Fusion sorter.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
1651 Page Mill
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Falstaff

Falstaff

PDF icon Falstaff Configuration

Falstaff is a 5-laser, 17-color sorter.  Its current laser/detector configuration provides 2-UV, 5-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

Falstaff Staff Assisted

PDF icon falstaffsept18-compatibilitymode.pdf

Falstaff is a 5-laser, 17-color sorter.  Its current laser/detector configuration provides 2-UV, 5-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD Aria ll
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm
Mikado

Mikado

is the most automated sorter that the Facility has. It is simple to use, and has tray and idex sorting. It is the best sorter for 384 trays, It has 3 lasers (405 nm, 488nm, 561nm) but is a little limited in fluorochrome choices as these lasers are co-linear,

Model: 
Sony SH800s
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
7500
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
405nm,488nm,561 nm

Mikado Email Only

is the most automated sorter that the Facility has. It is simple to use, and has tray and idex sorting. It is the best sorter for 384 trays, It has 3 lasers (405 nm, 488nm, 561nm) but is a little limited in fluorochrome choices as these lasers are co-linear

Model: 
Sony SH800s
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
7500
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
405nm,488nm,561 nm

Mikado startup

Book a 30 min slot ahead of your sort for staff to start-up the instrument

FREE of charge

You MUST provide the nozzle size needed for the experiment

Model: 
Sony
Room: 
B016
PICI

PICI

PDF icon PICI Configuration

PICI is a 5 laser, 18 color Aria Fusion sorter that was bought with Parker Institute for Cancer Immunotherapy funds.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Satchmo

Satchmo

PDF icon Satchmo Configuration

is a simple two-laser (488 nm and 640 nm) sorter. It is easy to operate, and does fast tray sorting, as well as index sorting. It uses the same polypropylene sample tubes as Carmen.

Model: 
BD FACSJazz
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
488 nm, 640 nm
Verdi

Verdi

PDF icon Verdi Configuration

Verdi is a 3-laser, 8-color sorter.  Its current laser/detector configuration provides 2-Blue, 3-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
7
Laser Excitations: 
488nm, 561nm, 640 nm

Instrument Specifics

Name Model Room Sort Clone Plate Reader Chiller / Heater Maximum cells / second Minutes to Sort 10[6] Cellssort descending BSL-2 Colors Laser Excitations Price per hour with no operator Configuration & Scheduling
Abbott Request Only BD LSR ll 1651 Page Mill Yes None 10000 Yes 17 405nm, 488nm, 633nm $58.00 PDF icon abbottsept18-compatibilitymode.pdf
Abbott Request Only
Symphony BD FACS Symphony A5 B003 No No Yes 10000 29 488nm, 532nm, 635nm, 405nm, 355nm $58.00 Symphony
Abbott BD LSR II 1651 Page Mill No No Yes 10,000 Yes 17 405nm, 488nm, 633nm $58.00 PDF icon Abbott Configuration
Abbott
Consultation- Main Facility $85.00 Consultation- Main Facility
Mikado startup Sony B016 $0.00 Mikado startup
Quanteon Acea Novocyte Quanteon B016C No Yes 20000 Yes 25 Blue, Violet, Yellow, Red $40.00 PDF icon Quanteon Configuration
Quanteon
Quanteon Sample Drop-off Acea Novocyte Quanteon B003 No Yes 20000 Yes 25 Blue, Violet, Yellow, Red $40.00 PDF icon Quanteon Configuration
Quanteon Sample Drop-off
Satchmo BD FACSJazz B016B Yes Yes No Chiller only 10,000 20 Yes 6 488 nm, 640 nm $50.00 PDF icon Satchmo Configuration
Satchmo
Verdi BD Aria II B016C Yes Yes No Both 25,000 3 Yes 7 488nm, 561nm, 640 nm $70.00 PDF icon Verdi Configuration
Verdi
Mikado Email Only Sony SH800s B019 Yes Yes No Chiller only 7500 35 Yes 6 405nm,488nm,561 nm $60.00 Mikado Email Only